pyrosequencing-based methylation analysis  (Pyrosequencing Inc)

Journal: PLoS ONE

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

doi: 10.1371/journal.pone.0056931

Figure Lengend Snippet: (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

Techniques: Methylation

Journal: PLoS ONE

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

doi: 10.1371/journal.pone.0056931

Figure Lengend Snippet: Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

Techniques: DNA Methylation Assay, Methylation

Journal: PLoS ONE

Article Title: Genome-Wide Hypomethylation in Head and Neck Cancer Is More Pronounced in HPV-Negative Tumors and Is Associated with Genomic Instability

doi: 10.1371/journal.pone.0004941

Figure Lengend Snippet: LINE-1 and Alu PMA Assays.

Article Snippet: To assay global methylation levels, we adapted our Pyrosequencing-based Methylation Analysis (PMA) assay to assess methylation of repetitive LINE and SINE ( Alu ) elements, similar to genome-wide methylation assays reported previously .

Techniques: