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pyrosequencing based analysis  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc pyrosequencing based analysis
    Pyrosequencing Based Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyrosequencing-based+analysis/16s+pyrosequencing+rrna/pm42128334-658-12-12
    Average 86 stars, based on 1 article reviews
    pyrosequencing based analysis - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Methylation:

    Article Title: Exploring methods to summarize gut microbiota composition for microbiability estimation and phenotypic prediction in swine.
    Article Snippet: Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines §.

    Article Title: Adiponectin Related Vascular and Cardiac Benefits in Obesity: Is There a Role for an Epigenetically Regulated Mechanism?
    Article Snippet: Pyrosequencing-based analysis was suitable for measuring subtle methylation changes at more than one CpG site and thus for detecting DNA methylation more accurately in the region ( – ).

    Article Title: Spectrum of BCR-ABL mutations in Azerbaijanian imatinib-resistant patients with chronic myeloid leukemia
    Article Snippet: During a 5.5 years Pyrosequencing-based analysis (January 2014 to May 2019) of BCR-ABL1 kinase domain mutations, at least one point mutation was identified in 22 of 163 patients (13.4%). shows the frequency of the mutations, as well as mutations leading to high levels of resistance to second-line TKIs [ ].

    Article Title: Multimodal magnetic resonance imaging (MRI) of juvenile and adult diffuse hemispheric gliomas with H3 G34-mutation: a case description
    Article Snippet: In addition, pyrosequencing-based analysis revealed a H3.3 G34R mutation ( ).

    Article Title: Targeting aberrant DNA hypermethylation as a driver of ATL leukemogenesis by using the new oral demethylating agent OR-2100.
    Article Snippet: The methylation status of CpG sites in the promoter proximal 23 region of LINE-1, THEMIS, LAIR1 and CD7 was determined using 24 D ow nloaded from https://ashpublications.org/blood/article-pdf/doi/10.1182/blood.2019003084/1728737/blood.2019003084.pdf by U PPSALA U N IVER SITETSBIBLIO TEK user on 22 M ay 2020 10 pyrosequencing-based analysis as described in detail in Supplemental Methods.

    Article Title: Adult T‐cell leukemia‐lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism
    Article Snippet: The methylation status of CpG sites in the promoter proximal regions of LINE‐1 and THEMIS was determined using pyrosequencing‐based analysis as described previously.

    Article Title: “Food-Omics” Applications In The Food Metagenom Profiling
    Article Snippet: Jung J., Choi S., Jeon C.O., Park W.: Pyrosequencing-based analysis of the bacterial community in Korean traditional seafood, ojingeo jeotgal.

    Article Title: Analysis of bacterial communities in sponges and coral inhabiting Red Sea, using barcoded 454 pyrosequencing
    Article Snippet: A total of 41,216 raw sequences were obtained from the sponges and coral samples from pyrosequencing-based analysis of 16S rRNA gene sequences.

    DNA Methylation Assay:

    Article Title: Exploring methods to summarize gut microbiota composition for microbiability estimation and phenotypic prediction in swine.
    Article Snippet: Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines §.

    Article Title: Adiponectin Related Vascular and Cardiac Benefits in Obesity: Is There a Role for an Epigenetically Regulated Mechanism?
    Article Snippet: Pyrosequencing-based analysis was suitable for measuring subtle methylation changes at more than one CpG site and thus for detecting DNA methylation more accurately in the region ( – ).

    Article Title: Spectrum of BCR-ABL mutations in Azerbaijanian imatinib-resistant patients with chronic myeloid leukemia
    Article Snippet: During a 5.5 years Pyrosequencing-based analysis (January 2014 to May 2019) of BCR-ABL1 kinase domain mutations, at least one point mutation was identified in 22 of 163 patients (13.4%). shows the frequency of the mutations, as well as mutations leading to high levels of resistance to second-line TKIs [ ].

    Article Title: Multimodal magnetic resonance imaging (MRI) of juvenile and adult diffuse hemispheric gliomas with H3 G34-mutation: a case description
    Article Snippet: In addition, pyrosequencing-based analysis revealed a H3.3 G34R mutation ( ).

    Article Title: Targeting aberrant DNA hypermethylation as a driver of ATL leukemogenesis by using the new oral demethylating agent OR-2100.
    Article Snippet: The methylation status of CpG sites in the promoter proximal 23 region of LINE-1, THEMIS, LAIR1 and CD7 was determined using 24 D ow nloaded from https://ashpublications.org/blood/article-pdf/doi/10.1182/blood.2019003084/1728737/blood.2019003084.pdf by U PPSALA U N IVER SITETSBIBLIO TEK user on 22 M ay 2020 10 pyrosequencing-based analysis as described in detail in Supplemental Methods.

    Article Title: Adult T‐cell leukemia‐lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism
    Article Snippet: The methylation status of CpG sites in the promoter proximal regions of LINE‐1 and THEMIS was determined using pyrosequencing‐based analysis as described previously.

    Article Title: “Food-Omics” Applications In The Food Metagenom Profiling
    Article Snippet: Jung J., Choi S., Jeon C.O., Park W.: Pyrosequencing-based analysis of the bacterial community in Korean traditional seafood, ojingeo jeotgal.

    Article Title: Analysis of bacterial communities in sponges and coral inhabiting Red Sea, using barcoded 454 pyrosequencing
    Article Snippet: A total of 41,216 raw sequences were obtained from the sponges and coral samples from pyrosequencing-based analysis of 16S rRNA gene sequences.



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    (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by <t>a</t> <t>pyrosequencing</t> based methylation assay of repetitive <t>DNA</t> elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).
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    (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

    Journal: PLoS ONE

    Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

    doi: 10.1371/journal.pone.0056931

    Figure Lengend Snippet: (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

    Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

    Techniques: Methylation

    Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

    Journal: PLoS ONE

    Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

    doi: 10.1371/journal.pone.0056931

    Figure Lengend Snippet: Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

    Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

    Techniques: DNA Methylation Assay, Methylation